Lactulose is a synthetic disaccharide composed of galactose and fructose. It is closely related to lactose which is a disaccharide consisting of galactose and glucose. Since Lactulose is not absorbed by the gut; it is therefore used as a laxative and for hepatic encephalopathy and is considered a prebiotic. It is detectable in heated milk since it is easily formed by isomerization of lactose at elevated temperatures or in alkaline solutions. Significant background is observed in traditional assays where glucose formed from the fructose is used to measure the lactulose concentration in the samples. BioVision’s Lactulose Assay Kit provides a simple, sensitive, & convenient assay to measure Lactulose. In this kit, fructose is directly measured using Lactulose Enzyme Mix after hydrolysis of Lactulose into galactose and fructose. The linear range of the assay is between approximately 10 pmol and 1 nmol.