The Link-FAST™ 5 minutes DNA Ligation Kit provides a fast and convenient means for ligation of sticky-end or blunt-end DNA fragments in just 5 minutes at room temperature. All necessary reagents required for ligation are provided. There is no need to prepare buffers or to add ATP or Mg²ᶧ. The kit can be used for cloning in plasmid vectors or phage vectors, linker ligation, recirculation of linear DNA, etc. Ligated DNA can be easily analyzed by agarose gel electrophoresis.