BioVision’s Human Angiopoietin-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-1. This assay employs an antibody specific for human Angiopoietin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiopoietin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of Angiopoietin-1 is typically less than 30 pg/ml. Detection Range: 30 pg/ml – 18000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.