Prolactin (PRL) is secreted from the anterior pituitary gland in both men and woman. Women normally have slightly higher basal prolactin levels than men. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications. Hyperprolactinemia inhibits hypogonadism in men and women with accompanying low FSH and LH levels. BioVision’s Prolactin kit is a solid phase direct sandwich ELISA Kit. The samples, and anti-Prolactin HRP conjugate are added to the wells coated with monoclonal antibody to Prolactin. Prolactin in the sample binds to anti-Prolactin Mab on the well and the anti-Prolactin HRP then binds to Prolactin. Unbound Protein and HRP conjugate is washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of Prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the Prolactin.