Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. BioVision’s rat VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat VEGF captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.