BioVision’s Progesterone ELISA kit is a competitive immunoassay for the quantitative determination of Progesterone in biological fluids. The kit uses a polyclonal antibody to Progesterone to bind, in a competitive manner, Progesterone in a sample or Progesterone which has an alkaline phosphatase molecule covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405 nm. The intensity of the bound yellow color is inversely proportional to the concentration of Progesterone in either standards or samples. The measured optical density for the samples is used to calculate the concentration of Progesterone in the sample. Sensitivity: 8.57 pg/ml (range: 15.62-500 pg/ml). The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Cross Reactivity: Progesterone:100%, 5-Pregnane-3,20-dione: 100%, 17-OH-Progesterone: 3.46%, 5 alpha-Pregnen-3ß-o1-20-one: 1.43%, Corticosterone: 0.77%, 4-Androstene-3,17-dione: 0.28%, Deoxycorticosterone: 0.056%, DHEA: 0.013%, 17ß-Estradiol: <0.001%, Estrone: 0.001%, Estriol: <0.001%, Testosterone: <0.001%, Hydrocortisone: <0.001%, 5 alpha-Pregnane-3,20-diol: <0.001%, Danazol: <0.001%.