BioVision’s Mouse Lipocalin-2 (LCN2)/NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Lipocalin-2. This assay employs an antibody specific for mouse Lipocalin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Lipocalin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Lipocalin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon. Sensitivity: 0.02 ng/ml. Detection Range: 0.02 ng/ml – 100 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.