KEY FEATURES:
• Sulfo-NHS-SS-Biotin is amine-reactive and
• Has a long chain spacer arm (24.3 Å) separating the biotin group from the amino-reactive sulfo-NHS ester
• Water-soluble; can be used under physiologic conditions
• It is possible to cleave the biotin group from the cross-linked target protein under reducing conditions, such as in the presence of BME, DTT or TCEP
APPLICATIONS:
• Sulfo-NHS-SS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein purification, detection or immobilization
• Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
• Sulfo-NHS-SS-Biotin is amine-reactive and
• Has a long chain spacer arm (24.3 Å) separating the biotin group from the amino-reactive sulfo-NHS ester
• Water-soluble; can be used under physiologic conditions
• It is possible to cleave the biotin group from the cross-linked target protein under reducing conditions, such as in the presence of BME, DTT or TCEP
APPLICATIONS:
• Sulfo-NHS-SS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein purification, detection or immobilization
• Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent