KEY FEATURES:
• Sulfo-LC-SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed
• Amine-reactive; the "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 Å spacer arm
• More water-soluble than the corresponding LC-SPDP
• Membrane-impermeable which enables crosslinking on cell surface
• The cleavable spacer arm enables separation of cross-linked products
APPLICATIONS:
• Reversible crosslinking of proteins
• N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains
• Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.
• Sulfo-LC-SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed
• Amine-reactive; the "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 Å spacer arm
• More water-soluble than the corresponding LC-SPDP
• Membrane-impermeable which enables crosslinking on cell surface
• The cleavable spacer arm enables separation of cross-linked products
APPLICATIONS:
• Reversible crosslinking of proteins
• N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains
• Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.