KEY FEATURES:
• SPDP contains a dithiopyridyl group which is reactive towards cysteines (-SH groups) and an NHS group, which is useful when targeting primary amines (-NH2 groups)
• SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed
• Contains a disulfide linkage in its spacer arm with a length of 6.8 Å
• The spacer is cleavable with reducing agent, such as DTT, TCEP, etc.
APPLICATIONS:
• Reversible crosslinking of proteins
• N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains
• Useful for adding sulfhydryl groups to proteins by attachment to lysine residues
• Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates
• SPDP contains a dithiopyridyl group which is reactive towards cysteines (-SH groups) and an NHS group, which is useful when targeting primary amines (-NH2 groups)
• SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed
• Contains a disulfide linkage in its spacer arm with a length of 6.8 Å
• The spacer is cleavable with reducing agent, such as DTT, TCEP, etc.
APPLICATIONS:
• Reversible crosslinking of proteins
• N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains
• Useful for adding sulfhydryl groups to proteins by attachment to lysine residues
• Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates