trans-Zeatin belongs to the plant hormone family known as ‘cytokinins’, which regulate cell division, development, and nutrient processing. The biosynthesis of cytokinins involves isoprenylation of ATP or ADP, with trans-Zeatin being produced by the removal of phosphate and riboside groups from adenine and oxidation by cytochrome P735A. Cytokininins, including trans-Zeatin are pivotal in governing overall plant size and can extend the plant photosynthesis by delaying leaf aging and death.