DAF-2 diacetate is a cell-permeable derivative of DAF-2 (Cat. No. 2752) and a very useful indicator of nitric oxide (NO). Upon entry into the cell, DAF-2 diacetate is converted into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.