A convenient set consisting of five Hedgehog (Hh) signaling pathway inhibitors and a negative control. The five inhibitors are: 50 µg of Cyclopamine-KAAD (Cat. No. 1910-50), 5 mg of GANT58 (Cat. No. 1812-5), 5 mg of GDC-0449 (Cat. No. 1890-5), 1 mg of Hh Signaling Pathway Antagonist (Cat. No. 1726-1), 1 mg of JK 184 (Cat. No. 1726-1). Also provided is 25 mg of Tomatidine hydrochloride (Cat. No. 1893-25) which can act as a negative control.