A convenient, sterile-filtered cocktail solution (1000X, in DMSO) containing three small molecules useful to induce rapid and efficient neuronal differentiation of human pluripotent stem cells (hPSCs) into nociceptors. The three products are: GSK-3beta inhibitor CHIR99021 (Cat. No. 1677), gamma-secretase inhibitor DAPT (Cat. No. 1855) and FGFR inhibitor SU-5402 (Cat. No. 1645).