Hi-BindTM Protein A-Agarose beads are specially prepared for high IgG binding by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein affinity purification methods. BioVision’s Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A than the other regular Protein-A Agarose on the market. The IgG binding capacity of Hi-BindTM Protein A-Agarose is ≥ 36 mg human or rabbit IgG per ml of wet beads. The Hi-BindTM Protein A-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.