Biotinylated BSA is synthesized through biotinylation of BSA with Sulfo-NHS-LC-Biotin (Cat # 2326). It has 3 moles biotin/mole of BSA as determined by Biovision’s Biotin Estimation Kit, Catalog # K811-50, 100. It can be used as a positive control in biotin estimation procedures and as a competitive inhibitor for anti-biotin antibodies.