pVisionRFP-C is an eukaryotic (mammalian) expression vector encoding red fluorescent protein VisionRFP from sea anemone Entacmaea quadricolor. The vector allows to generate fusions to the VisionRFP C-terminus and to express VisionRFP fusions or VisionRFP alone in eukaryotic (mammalian) cells.
pVisionRFP-C vector carries synthetic VisionRFP gene which codon usage is humanized, i.e. optimized for high expression in mammalian cells. pVisionRFP-C vector contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication and neomycin resistance (Neor) gene for selection in eukaryotic cells. It also contains pUC origin of replication for propagation in E. coli and f1 origin for single-stranded DNA production. Bacterial promoter (P) provides expression of kanamycin resistance gene in E. coli. To increase VisionRFP translation, Kozak consensus translation initiation site is generated upstream of VisionRFP sequence. Multiple cloning site (MCS) is between VisionRFP coding sequence and the SV40 polyadenylation signal (SV40 poly A).
pVisionRFP-C vector carries synthetic VisionRFP gene which codon usage is humanized, i.e. optimized for high expression in mammalian cells. pVisionRFP-C vector contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication and neomycin resistance (Neor) gene for selection in eukaryotic cells. It also contains pUC origin of replication for propagation in E. coli and f1 origin for single-stranded DNA production. Bacterial promoter (P) provides expression of kanamycin resistance gene in E. coli. To increase VisionRFP translation, Kozak consensus translation initiation site is generated upstream of VisionRFP sequence. Multiple cloning site (MCS) is between VisionRFP coding sequence and the SV40 polyadenylation signal (SV40 poly A).